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Distal downstream signaling and IR It is generally accepted that diverse downstream targets of Akt activation lead to different distal signaling in target tissues response to insulin

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Cell viability assay Cells were seeded at 2 × 10³ cells per well in 96-well plates and differentiated for 8 days with RA, while being subjected to FeSO₄ or BSO under chronic (9-day continuous exposure with media replacement every 2 days) or acute (8-h exposure on day 9) conditions