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We found consistent downregulation of GPX3 expression by TNF-α in both cell types, both on transcript and on secreted protein level (Figs 7A and 8A)

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In vitro treatment of TMAO together with pro-inflammatory cytokine TNF-α can increase the proliferation (detected by the cell-counting Kit-8 assay), migration (detected by the wound healing assay and the transwell assay), and invasion [detected by the expression levels of periostin, integrin-linked kinase (ILK)/RAC-α serine/threonine-protein kinase (AKT1), and the mammalian target of rapamycin (mTOR)] of mouse liver cancer cell line Hepa1–6 cells and human liver cancer cell line Huh7 cells [64]