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A comparative time-course study by Shadrina, Kolomin, and colleagues in 2010, published in the Journal of Molecular Neuroscience , characterized NGF and BDNF gene expression in rat hippocampus, frontal cortex, and retina under Semax, and a 2010 paper by Dmitrieva, Povarova, and colleagues in Cellular and Molecular Neurobiology showed that Semax and its C-terminal Pro-Gly-Pro fragment activate neurotrophin and receptor transcription after cerebral ischemia, directly linking the BDNF and NGF mechanism to the stroke indication