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In addition to this protein–protein interaction, Thi2.4 exhibits direct antifungal activity by disrupting fungal cell membranes ( 2.1.3.3 Insect and nematode resistance In barley ( Hordeum vulgare ), the thionin genes THIO1567 and THIO1570 exhibited significantly higher transcript levels in the bird cherry–oat aphid ( Rhopalosiphum padi L.)-resistant genotype Hsp5 compared to the susceptible genotype Lina, suggesting a role in aphid resistance ( Oryza sativa ) than in the resistant accession TOG5681U ( Oryza glaberrima ) following nematode infection, indicating a potential contribution of thionins to nematode resistance (Petitot et al., 2017)

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