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Tissue processing Following the 3 weeks of cuprizone treatment, mice were euthanised using pentobarbitone sodium (160 mg/kg, Delvet), and transcardially perfused with 0.9% saline, followed by 4% paraformaldehyde (Sigma-Aldrich) in 0.1 M phosphate buffered solution pH 7.4

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These animals also received an intraperitoneal dose of deuterated saline targeted to enrich TBW to 5% 2 H 2 O and were offered 5% 2 H 2 O as drinking water to maintain the TBW enrichment