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Elevated ROS production in urothelial cells has been associated with upregulation of the transient receptor potential cation channel subfamily M member 8 (TRPM8), which is associated with increased frequency of bladder emptying, increased levels of inducible nitric oxide synthase, peroxidation of membrane phospholipids, MDA formation, decreased total antioxidant capacity, as well as ultrastructural alterations in mitochondria with accumulation of lipofuscin [159, 160]