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Encouraged by the gain-of-function from Glu72Arg, we searched for additional mutations enriched during the evolution of X(PC4) phages on the GD_M substrate (Supplementary Table 5) with the goal of improving activity so that proteolysis would be detectable in mammalian cells

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Distinct cysteine residues in Keap1 are required for Keap1-dependent ubiquitination of Nrf2 and for stabilization of Nrf2 by chemopreventive agents and oxidative stress