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In the substrate oxidation stress test, the cells were prepared as above prior to the assay, and the cells were subjected to (in sequence, in final concentrations): (1) 0, 4 mM l-glutamine (Seahorse Bioscience, Billerica, MA, USA), 40 μM Etomoxir (11969, Cayman Chemical, Ann Arbor, USA) or 3 μM BPTES ((bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide)

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Glutaminolysis, a vital metabolic process in cancer cells that involves the breakdown of Gln, is essential for the synthesis of biomacromolecules necessary for supporting biosynthetic requirements, including proteins, fatty acids, and nucleotides