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Cells were washed again twice with 1× Perm/Wash Buffer (BD Biosciences), re-suspended in PBS and subjected to flow cytometric analysis at an excitation wavelength of (488 nm) employing BD FACSymphony™ A1 Cell Analyzer (Becton Dickinson, San Jose, CA, USA) equipped with BD FACSDiva™ Software v9.0.2

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[DOI] [PMC free article] [PubMed] [Google Scholar] 178.Breheny D., Adamson J., Azzopardi D., Baxter A., Bishop E., Carr T., Crooks I., Hewitt K., Jaunky T., Larard S., et al