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After denaturation (2 min at 94°C) 18 cycles were conducted, consisting of 45 s at 94°C, 30 s at 55°C, and 15 min at 72°C, followed by a final extension step at 72°C for 10 min

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The idea of the anti-inflammatory properties of vitamin D has been further supported by a clinical study that reported how concentrations of IL-1β, IL-6, interleukin-8, macrophage inflammatory protein 1β, monocyte chemoattractant protein-1, and IFN-inducible protein 10 markedly decreased after co-treatment with vitamins D and B12