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A piperonyl butoxide (PBO) synergist assay was used to test the involvement of cytochrome P450s , while qRT-PCR quantified expression of detoxification genes ( CYP6P1 , CYP9K1 , CYP6M2 , CYP6P3 , CYP4G16 , GSTE2 , and CYP6Z1 )