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discovered berberine as a novel negative regulator of PD-L1 in NSCLC, it exerted antitumor effect through diminishing PD-L1 expression via specific binding to Glu76 of CSN5 and inhibiting its deubiquitination, as well as activating tumor-infiltrating T cells, which provided a theoretical basis for the potential application of berberine as a small-molecule inhibitor to disrupt PD-L1-mediated immunosuppression