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The resulting protein pellets were dissolved in 200 µL of 8 M urea in 50 mM Tris/HCl (pH 8.0) and heat-denatured at 95°C for 10 min

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25 A survey in the UK, by Seage and Semedo, investigated the impact on people with pernicious anaemia (n=329) who experienced a suspension of injections, with tablets being offered as an alternative