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As described more particularly herein, proteins regulate diverse cellular processes in higher organisms, ranging from rapid metabolic changes to growth and differentiation

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Next, 100 μM TAMRA-PEG 3 -N 3 , 200 μM Cu(II)SO 4 , 1 mM THPTA and 5 mM freshly dissolved sodium-ascorbate were added to the mixture and it was further incubated at ambient temperature, under constant agitation (300 rpm) for overnight